Oral Presentation Australasian Cytometry Society 2016 Annual Conference

Drop-based Microfluidic cell sorting (24099)

David Weitz 1
  1. Harvard University, Cambridge, MASSACHUSETTS, United States

This talk will describe the use aqueous drops, immersed in an inert carrier oil, as picoliter-volume containers for single cells.  The cells can be manipulated in the drops, and assays can be performed on the cells while confined within the drops.  Because excreted molecules remain trapped within the drop, there is no need to chemically bind the molecule to the cell.  The cell-containing drops can be sorted at rates that are comparable to those achieved with FACS, allowing large numbers of cells to be screened and chosen cells to be selected.  Applications to single-cell sequencing will be highlighted.